Ferry Efendi

Berbagi, Peduli dan Menginspirasi

Searching for the best agarose candidate from genus Gracilaria, Eucheuma, Gelidium and local brands

01 July 2017 - dalam Publikasi Internasional Terindeks Scopus Oleh ferry-efendi-fkp

FerryEfendi1. Numbers and letters correspond to the affiliation list. Click to expose these in author workspaceOpens the author workspaceOpens the author workspace. Author links open the author workspace.RetnoHandajani2. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace3. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace. Author links open the author workspace.NursalamNursalam1. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace

1
    Faculty of Nursing, Airlangga University, Surabaya, Indonesia

2
    Department of Biochemistry Medicine, Faculty of Medicine, Airlangga University, Surabaya, Indonesia

3
    Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia

https://doi.org/10.1016/j.apjtb.2015.06.009
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Open Access funded by Hainan Medical University
Under a Creative Commons license
open access
Abstract
Objective

To explore the potential of local agar of genus Gracilaria, Eucheuma, Gelidium and local brands as an alternative for imported agarose for DNA electrophoresis, and to examine their ability related to separation and migration of DNA fragments in DNA electrophoresis.
Methods

Their performance at various concentrations were compared via an experimental study with a specific brand of imported commercial agarose used in molecular biology research. The measured variables were separation and migration during electrophoresis of a DNA fragment.
Results

The local agar genus Gracilaria gigas, Gelidium, brand “B” and brand “S” could separate DNA fragments at a concentration between 1% and 2%, with an optimum concentration of 2% w/v, as good as a specific brand of imported commercial agarose.
Conclusions

Their performance were very close to that of commercial agarose and can still be improved by further agar purification as well as by pH and sulfur control.
Keywords
Agarose
Eucheuma
Gracilaria
Gelidium
Local brands
Indonesia agar



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